Effects of Functional Protein on Osteoblasts in Rat
نویسنده
چکیده
To assess the effects of functional protein on osteoblast, Large quantity of high-purity osteoblasts had been cultivated successfully by adopting sequential enzyme digestion. The growth curve of osteoblasts was protracted by cell counting. Proliferation of osteoblasts was assessed by MTT colorimetry. The experimental results show the functional protein can enhance proliferation, the properties of adhesion and discuss the effect of osteopontin on osteoblast. Keywords—functional protein; osteoblast; MTT
منابع مشابه
Induction of Mineralized Nodule Formation in Rat Bone Marrow Stromal Cell Cultures by Silk Fibroin
Background: Silk fibroin is a suitable protein for osteogenesis by inducing markers of bone formation in the cultures of osteoblasts, so we examined the ability of this protein to induce mineralized nodules in the rat bone marrow stromal cell cultures. Methods: Bone marrow stromal cells obtained from 4 to 6 weeks old Spruge-Dawely male rats were grown in primary culture for seven days and then ...
متن کاملTriiodothyronine potentiates angiogenesis-related factor expression through PI3K/AKT signaling pathway in human osteoarthritic osteoblasts
Objective(s): Previous study has indicated that triiodothyronine (T3) facilitated cartilage degeneration in osteoarthritis (OA). This study aimed to investigate the effects of T3 on angiogenesis-related factor expression in human osteoblasts of OA subchondral bone.Materials and Methods: The subchondral bone specimens were obtained from O...
متن کاملDifferential Effects of Resveratrol on the Expression of Brain-Derived Neurotrophic Factor Transcripts and Protein in the Hippocampus of Rat Brain
Background: The induction of brain-derived neurotrophic factor (BDNF) expression in the hippocampus has shown to play a role in the beneficial effects of resveratrol (RSV) on the learning and memory. The BDNF gene has a complicated structure with eight 5’ noncoding exons (I-IXa), each of which can splice to a common coding exon (IX) to form a functional transcript. Estrogens increase levels of ...
متن کاملEffects of silver nanoparticles on the functional tests of liver and its histological changes in adult male rats
Objective(s): Silver nanoparticles show anti-fungal properties, and is widely used in medicine. In this research, the impacts of silver nanoparticles on the hepatic functional tests and changes in liver tissues in adult male rats were investigated. Methods: In this experimental study, 28 adult male Wistar rats, each weighing approximately 180-220 g were divide...
متن کاملEffects of lipopolysaccharide-induced septic shock on rat isolated kidney, possible role of nitric oxide and protein kinase C pathways
Objective(s): Pathophysiology of sepsis-associated renal failure (one of the most common cause of death in intensive care units) had not been fully determined. The effect of nitric oxide and protein kinase C (PKC) pathways in isolated kidney of Lipopolysaccharide-treated (LPS) rats were investigated in this study. Materials and Methods: Vascular responsiveness to phenylephrine and acetylcholine...
متن کامل